804g bladder carcinoma cell line Search Results


804g bladder carcinoma cell line  (ViaCyte Inc)
90
ViaCyte Inc 804g bladder carcinoma cell line
Glucose- and calcium-mediated activation of ATP synthase-dependent respiration in human islets. Human islets bound to <t>804G</t> matrix were incubated in KRBH ( A ) or a KRBH buffer lacking Ca 2+ supplemented with 0.4 m m EGTA ( B ; Ca 2+ -free). For each condition the mean ± S.E. n = 3 from the same donor is shown. Total islet protein varied between wells (4–6 μg). Because of these variations the results are expressed relative to the respiratory rate before glucose stimulation. ATP synthase-dependent ( dep , C ) and -independent respiration ( D ) was quantified as described under “Experimental Procedures.” The results are the mean ± S.E. ( n = 6) obtained from 2 donors. *, p < 0.01; **, p < 0.001; ns , not significant. R , rotenone (1 μ m ); AA , antimycin A (1 μg/ml); O , oligomycin (2.5 μg/ml); glc , glucose. E , preventing calcium signaling blocks glucose-induced insulin secretion. Insulin secretion from human islets was determined in KRBH or the same buffer lacking Ca 2+ in either 1 m m ( gray bars ) or 16.7 m m glucose ( black bars ). Shown is the average ±S.E. n = 4 result with islets from a single donor (*, p < 0.05).
804g Bladder Carcinoma Cell Line, supplied by ViaCyte Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Glucose- and calcium-mediated activation of ATP synthase-dependent respiration in human islets. Human islets bound to 804G matrix were incubated in KRBH ( A ) or a KRBH buffer lacking Ca 2+ supplemented with 0.4 m m EGTA ( B ; Ca 2+ -free). For each condition the mean ± S.E. n = 3 from the same donor is shown. Total islet protein varied between wells (4–6 μg). Because of these variations the results are expressed relative to the respiratory rate before glucose stimulation. ATP synthase-dependent ( dep , C ) and -independent respiration ( D ) was quantified as described under “Experimental Procedures.” The results are the mean ± S.E. ( n = 6) obtained from 2 donors. *, p < 0.01; **, p < 0.001; ns , not significant. R , rotenone (1 μ m ); AA , antimycin A (1 μg/ml); O , oligomycin (2.5 μg/ml); glc , glucose. E , preventing calcium signaling blocks glucose-induced insulin secretion. Insulin secretion from human islets was determined in KRBH or the same buffer lacking Ca 2+ in either 1 m m ( gray bars ) or 16.7 m m glucose ( black bars ). Shown is the average ±S.E. n = 4 result with islets from a single donor (*, p < 0.05).

Journal: The Journal of Biological Chemistry

Article Title: Calcium Co-regulates Oxidative Metabolism and ATP Synthase-dependent Respiration in Pancreatic Beta Cells

doi: 10.1074/jbc.M113.513184

Figure Lengend Snippet: Glucose- and calcium-mediated activation of ATP synthase-dependent respiration in human islets. Human islets bound to 804G matrix were incubated in KRBH ( A ) or a KRBH buffer lacking Ca 2+ supplemented with 0.4 m m EGTA ( B ; Ca 2+ -free). For each condition the mean ± S.E. n = 3 from the same donor is shown. Total islet protein varied between wells (4–6 μg). Because of these variations the results are expressed relative to the respiratory rate before glucose stimulation. ATP synthase-dependent ( dep , C ) and -independent respiration ( D ) was quantified as described under “Experimental Procedures.” The results are the mean ± S.E. ( n = 6) obtained from 2 donors. *, p < 0.01; **, p < 0.001; ns , not significant. R , rotenone (1 μ m ); AA , antimycin A (1 μg/ml); O , oligomycin (2.5 μg/ml); glc , glucose. E , preventing calcium signaling blocks glucose-induced insulin secretion. Insulin secretion from human islets was determined in KRBH or the same buffer lacking Ca 2+ in either 1 m m ( gray bars ) or 16.7 m m glucose ( black bars ). Shown is the average ±S.E. n = 4 result with islets from a single donor (*, p < 0.05).

Article Snippet: The 804G bladder carcinoma cell line ( ) and protocols to isolate the matrix were obtained from Viacyte (San Diego, CA).

Techniques: Activation Assay, Incubation